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wEstErn BlotϴĤ

ϴĤϵ׵ķԽ,Խϵ.

ԭϲƼ.ΪɫĤϽϵĶ,ø,ͨø뻯ѧﷴӦ,ź,Ӷعɵý.һ,øĤϵĵ׿ܻή,ķӦԱ,ʧ,пʵʧܵ.һĤԺ,Ҳпܻı.Բ鳤ʱ䲻к.

ʲôWesternblot? Ѵ Westernӡ(Western Blot)ǽתƵĤ,Ȼÿм.֪ﵰ,ӦΪһм,

Ҫ.stripping bufferǿ,ԭ-Ľ϶ϴ.մϵǵ㶫,ɾϴ.

512KD?̫һ.ת200KD,Կתҹ? ȷʵwestern,ApoB10048,תĤʱҪܳܳ,ǵ

1.ƷŨȵ,Ũ(Ҫ̫).2.Ƿ̫Сܶ,ӷ뽺Ũ,̵Ӿʱ.3.ԺʲôϴĤ?ֻõTBST?ҶTBSTϴTBS(ȥµTBST)ϴ.԰,ξ֪,ʵʱȶ.

Western Blotͨ۱ϩӾ,PAGEĵƷ,תƵ(άرĤ),Էǹۼʽ,ֵܱӾĶͼѧԲ.Թϵĵ

NCĤ Ĥ PVDFĤ Ⱥͷֱ ϸ 80-110 ug/cm2 >400 ug/cm2 125-200 ug/cm2 (ʺSDS뵰ʵĽ) ʵ ɵNCĤ״ ʵ еǿȸ ܼ Һʪ, Һʪ ʹǰ100%״ʪ ⷽʽ Ⱦɫ,÷ԺͷǷԼ Ⱦ Ⱦɫ,ÿ˹Ⱦɫ ÷Χ 0.45um һ㵰 0.2umһС20kD 0.1umһС7kD ۸ ۸ϱ Ϲ

ϴĤ.ڷյʱǿзյ,ϴдķ,ϴӿ԰ѽϵIJǺι̵һЩϴȥ.,ڲоĤ,߱.

ûоʵûоֱϴ ˵Ҳ Ƚ϶ľ

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